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antibody against calbindin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antibody against calbindin
    Antibody Against Calbindin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibody+against+calbindin/pm41703050-252-36-39
    Average 86 stars, based on 1 article reviews
    antibody against calbindin - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: GGC repeat expansions within new open reading frames are translated into toxic polyglycine proteins in oculopharyngodistal myopathy.
    Article Snippet: .. Slides were blocked for 1 h with PBS, 0.5% Triton X-100 and 5% horse serum for immunofluorescence of PBS with 0.1% Tween-20 and 5% BSA for immunochemistry followed by overnight incubation at 4 °C with primary antibody against Calbindin (Cell Signaling Technology, 13176S; 1:800), GFAP (Abcam, ab68428; 1:10,000), p62 (Abcam, ab56416; 1:1,000), p62 (Cell Signaling Technology, 23214S; 1:500) or tyrosine hydroxylase (Abcam, ab112; 1:2,000). .. For immunofluorescence, slides were washed with PBS containing 0.1% Triton X-100, incubated with donkey antimouse or donkey antirabbit secondary antibodies conjugated with Alexa 488 or CY3 ( Jackson Immunoresearch; 1:500) for 1 h, washed twice with PBS containing 0.1% Triton X-100 and incubated for 3 min in PBS/DAPI (1:1,000 dilution).

    Incubation:

    Article Title: GGC repeat expansions within new open reading frames are translated into toxic polyglycine proteins in oculopharyngodistal myopathy.
    Article Snippet: .. Slides were blocked for 1 h with PBS, 0.5% Triton X-100 and 5% horse serum for immunofluorescence of PBS with 0.1% Tween-20 and 5% BSA for immunochemistry followed by overnight incubation at 4 °C with primary antibody against Calbindin (Cell Signaling Technology, 13176S; 1:800), GFAP (Abcam, ab68428; 1:10,000), p62 (Abcam, ab56416; 1:1,000), p62 (Cell Signaling Technology, 23214S; 1:500) or tyrosine hydroxylase (Abcam, ab112; 1:2,000). .. For immunofluorescence, slides were washed with PBS containing 0.1% Triton X-100, incubated with donkey antimouse or donkey antirabbit secondary antibodies conjugated with Alexa 488 or CY3 ( Jackson Immunoresearch; 1:500) for 1 h, washed twice with PBS containing 0.1% Triton X-100 and incubated for 3 min in PBS/DAPI (1:1,000 dilution).

    Binding Assay:

    Article Title: Nrf2 Activation Does Not Protect from Aldosterone-Induced Kidney Damage in Mice.
    Article Snippet: .. After visualization of antibody binding, the protocol was repeated with an antibody against calbindin (1:200, #2173, Cell Signaling, Herts, UK) to identify distal tubular cells and cells of the early collecting duct. ..

    Article Title: Nrf2 Activation Does Not Protect from Aldosterone-Induced Kidney Damage in Mice
    Article Snippet: .. After visualization of antibody binding, the protocol was repeated with an antibody against calbindin (1:200, #2173, Cell Signaling, Herts, UK) to identify distal tubular cells and cells of the early collecting duct. ..

    other:

    Article Title: GGC repeat expansions within new open reading frames are translated into toxic polyglycine proteins in oculopharyngodistal myopathy
    Article Snippet: Proteins were directly loaded on nitrocellulose membranes (Amersham Protan), washed twice with Towbin buffer, blocked with 5% nonfat dry milk in TBS-T and incubated with anti-GFP (Abcam, ab290; 1:10,000) in TBS-T with 5% nonfat dry milk overnight at 4 °C.



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    A. Representative images of Iba1+ cells in 34-week mouse lobule X. B. Quantification of microglia density. N=5 WT 2Q/2Q , N=5 f-ATXN1 146Q/2Q , and N=5 ATXN1 mKO mice 34–36 weeks of age. C. Representative Imaris 3D reconstruction images in cerebellar lobule X. D. Lobule X granular layer total filament length. E. Lobule X molecular layer total filament length. F. Lobule X granular layer soma volumes. G. Lobule X molecular layer soma volumes. H. Lobule X granular layer number of terminal points. I. Lobule X molecular layer number of terminal points. J. Heatmap of sensome genes. Measured using z-score. K. Representative images of GFAP intensity in 32–35-week-old mouse lobule X. L. Quantification of GFAP intensity. N=6 WT 2Q/2Q , N=6 f-ATXN1 146Q/2Q , and N=6 ATXN1 mKO mice 32-–35 weeks of age. M. Heatmaps of 35-week cerebellar A1 gene expression ( Top ) and A2 gene expression ( Bottom ). N. Representative images of VGLUT2, <t>Calbindin,</t> and merged intensities. O. Quantification of Calbindin intensity. N=5 WT 2Q/2Q , N=5 f-ATXN1 146Q/2Q , and N=5 ATXN1 mKO mice 32-35 weeks of age. P. Quantification of molecular layer width. N=6 WT 2Q/2Q , N=6 f-ATXN1 146Q/2Q , and N=6 ATXN1 mKO mice 32–35 weeks of age. Q. Quantification of VGLUT2 width over molecular layer width. N=4 WT 2Q/2Q , N=4 f-ATXN1 146Q/2Q , and N=4 ATXN1 mKO mice 32–35 weeks of age. R. 35-week cerebellar magenta gene expression. 214 genes that were significantly altered in f-ATXN1 146Q/2Q and ATXN1 mKO as compared to WT. Data is average ± SEM with individual mice presented as dots. Open dots represent female mice while filled dots represent male mice. One-way ANOVA with Tukey’s test. * P < 0.05, ** P < 0.01, *** P < 0.001.
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    Image Search Results


    A. Representative images of Iba1+ cells in 34-week mouse lobule X. B. Quantification of microglia density. N=5 WT 2Q/2Q , N=5 f-ATXN1 146Q/2Q , and N=5 ATXN1 mKO mice 34–36 weeks of age. C. Representative Imaris 3D reconstruction images in cerebellar lobule X. D. Lobule X granular layer total filament length. E. Lobule X molecular layer total filament length. F. Lobule X granular layer soma volumes. G. Lobule X molecular layer soma volumes. H. Lobule X granular layer number of terminal points. I. Lobule X molecular layer number of terminal points. J. Heatmap of sensome genes. Measured using z-score. K. Representative images of GFAP intensity in 32–35-week-old mouse lobule X. L. Quantification of GFAP intensity. N=6 WT 2Q/2Q , N=6 f-ATXN1 146Q/2Q , and N=6 ATXN1 mKO mice 32-–35 weeks of age. M. Heatmaps of 35-week cerebellar A1 gene expression ( Top ) and A2 gene expression ( Bottom ). N. Representative images of VGLUT2, Calbindin, and merged intensities. O. Quantification of Calbindin intensity. N=5 WT 2Q/2Q , N=5 f-ATXN1 146Q/2Q , and N=5 ATXN1 mKO mice 32-35 weeks of age. P. Quantification of molecular layer width. N=6 WT 2Q/2Q , N=6 f-ATXN1 146Q/2Q , and N=6 ATXN1 mKO mice 32–35 weeks of age. Q. Quantification of VGLUT2 width over molecular layer width. N=4 WT 2Q/2Q , N=4 f-ATXN1 146Q/2Q , and N=4 ATXN1 mKO mice 32–35 weeks of age. R. 35-week cerebellar magenta gene expression. 214 genes that were significantly altered in f-ATXN1 146Q/2Q and ATXN1 mKO as compared to WT. Data is average ± SEM with individual mice presented as dots. Open dots represent female mice while filled dots represent male mice. One-way ANOVA with Tukey’s test. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: bioRxiv

    Article Title: Mutant ATXN1 impacts human and mouse microglia and contributes to cognitive, mood, and motor deficits in SCA1 mice

    doi: 10.64898/2026.02.10.705104

    Figure Lengend Snippet: A. Representative images of Iba1+ cells in 34-week mouse lobule X. B. Quantification of microglia density. N=5 WT 2Q/2Q , N=5 f-ATXN1 146Q/2Q , and N=5 ATXN1 mKO mice 34–36 weeks of age. C. Representative Imaris 3D reconstruction images in cerebellar lobule X. D. Lobule X granular layer total filament length. E. Lobule X molecular layer total filament length. F. Lobule X granular layer soma volumes. G. Lobule X molecular layer soma volumes. H. Lobule X granular layer number of terminal points. I. Lobule X molecular layer number of terminal points. J. Heatmap of sensome genes. Measured using z-score. K. Representative images of GFAP intensity in 32–35-week-old mouse lobule X. L. Quantification of GFAP intensity. N=6 WT 2Q/2Q , N=6 f-ATXN1 146Q/2Q , and N=6 ATXN1 mKO mice 32-–35 weeks of age. M. Heatmaps of 35-week cerebellar A1 gene expression ( Top ) and A2 gene expression ( Bottom ). N. Representative images of VGLUT2, Calbindin, and merged intensities. O. Quantification of Calbindin intensity. N=5 WT 2Q/2Q , N=5 f-ATXN1 146Q/2Q , and N=5 ATXN1 mKO mice 32-35 weeks of age. P. Quantification of molecular layer width. N=6 WT 2Q/2Q , N=6 f-ATXN1 146Q/2Q , and N=6 ATXN1 mKO mice 32–35 weeks of age. Q. Quantification of VGLUT2 width over molecular layer width. N=4 WT 2Q/2Q , N=4 f-ATXN1 146Q/2Q , and N=4 ATXN1 mKO mice 32–35 weeks of age. R. 35-week cerebellar magenta gene expression. 214 genes that were significantly altered in f-ATXN1 146Q/2Q and ATXN1 mKO as compared to WT. Data is average ± SEM with individual mice presented as dots. Open dots represent female mice while filled dots represent male mice. One-way ANOVA with Tukey’s test. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: We used the primary antibody Iba1 (rabbit, Abcam, Cat. No. AB107159), primary antibodies against Purkinje cell marker calbindin (rabbit, Cell Signaling Technology, Cat. No. D1I4Q), vesicular glutamate transporter 2 (VGLUT2) (guinea pig, Millipore, Cat. No. AB2251-I), astrocytic marker glial fibrillary acidic protein (GFAP) (chicken, Millipore, Cat. No. AB5541) as previously described ( ).

    Techniques: Gene Expression